Arizona Andrology Laboratory & Cryobank
Providing state-of-the-art male fertility testing, processing, cryopreservation, and storage

Gateway Wellness Center - 850 N. Kolb Road, Tucson, AZ 85710

Phone: 520-885-2689 - Fax: 520-731-0715

    

Vital Stain
    
Sperm that are non-motile are not necessarily “dead”. Many of these non-motile sperm are still alive and viable, and can be used for ART (Assisted Reproductive Technology) procedures such as ICSI (Intracytoplasmic Sperm Injection). In addition, the percentage of sperm that are viable but non-motile may indicate other clinical evidence of partial blockage or other factors that may render the sperm immotile, but still alive. The vital stain also provides an accurate check of the motile percentage of the specimen, since the percentage “alive” should equal or exceed the percentage motile. The vital stain is based on the principle that dead cells will take up an eosin dye, and as a result stain red. Live sperm do not take up the dye and remain colorless. The nigrosin provides a dark background which makes it easier to asses the slide.
    
    
    
    
    
    

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